Phospho-ESRα-S118 Rabbit Monoclonal Antibody (CABP1004)
- SKU:
- CABP1004
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Product Name: | Phospho-ESRα-S118 Rabbit Monoclonal Antibody |
| SKU: | CABP1004 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Mouse |
| Immunogen: | A synthetic phosphorylated peptide around S118 of human Estrogen Receptor alpha (ESR1) (P03372). |
| Sequence: | QLSP F |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | ER; ESR; Era; ESRA; ESTRR; NR3A1; Phospho-ESRα-S118 |
| Positive Sample: | NIH/3T3+EGF |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic side, Golgi apparatus, Nucleus, Peripheral membrane protein, Single-pass type I membrane protein. |
| Calculated MW: | 66kDa |
| Observed MW: | 66kDa |
This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.
| Purification Method: | Affinity purification |
| Gene ID: | 2099 |
| Clone Number: | ARC1549 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ESRα-S118 Rabbit mAb (CABP1004) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min. |
Related Products
ESRα Rabbit Monoclonal Antibody (CAB12976)
Phospho-p53-S392 Rabbit Monoclonal Antibody (CABP1137)
Phospho-mTOR-S2448 Rabbit Monoclonal Antibody (CABP0115)
Phospho-Smad2-S250 Rabbit Monoclonal Antibody (CABP1007)
