The Phospho-ERK3 (S189) Polyclonal Antibody (CABP0051) is a valuable tool for researchers studying ERK3, a protein kinase that plays a key role in cell signaling pathways. This antibody, generated in rabbits, specifically targets phosphorylated ERK3 at Serine 189, allowing for highly specific detection in Western blot analyses. With a high reactivity towards human samples, this antibody is ideal for investigating the phosphorylation status of ERK3 in various biological contexts.ERK3 is a member of the MAPK family and is involved in regulating processes such as cell proliferation, differentiation, and apoptosis. Phosphorylation at Serine 189 is known to impact the activity and function of ERK3, making this antibody crucial for studies aiming to dissect the signaling mechanisms controlled by this kinase.
Researchers interested in cancer biology, cell signaling, and kinase signaling pathways will find this antibody indispensable for their investigations.By utilizing the Phospho-ERK3 (S189) Polyclonal Antibody, researchers can gain valuable insights into the role of ERK3 in cellular processes and potentially identify novel therapeutic targets for diseases where dysregulation of ERK3 signaling is implicated. This antibody is a reliable tool for unlocking the complex functions of ERK3 and advancing our understanding of its involvement in health and disease.
Product Name:
Phospho-ERK3-S189 Rabbit Polyclonal Antibody
SKU:
CABP0051
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S189 of human ERK3 (NP_002739.1).
Sequence:
HLSE G
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
ERK3; PRKM6; p97MAPK; HsT17250; Phospho-ERK3-S189
Positive Sample:
293
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
83kDa
Observed MW:
120kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Purification Method:
Affinity purification
Gene ID:
5597
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody (CABP0051) at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
