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Phospho-ERK3-S189 Rabbit Polyclonal Antibody (CABP0051)

SKU:
CABP0051
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-ERK3-S189 Rabbit Polyclonal Antibody
SKU:CABP0051
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S189 of human ERK3 (NP_002739.1).
Sequence:HLSE G
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:ERK3; PRKM6; p97MAPK; HsT17250; Phospho-ERK3-S189
Positive Sample:293
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:83kDa
Observed MW:120kDa

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Purification Method:Affinity purification
Gene ID:5597
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody (CABP0051) at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.

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