The Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187) Polyclonal Antibody (CABP0472) is a valuable tool for researchers studying the MAPK/ERK signaling pathway. This antibody, raised in rabbits, is highly specific to phosphorylated ERK1 (T202/Y204) and ERK2 (T185/Y187) and is validated for use in Western blot applications. It enables detection and quantification of phosphorylated ERK1 and ERK2, allowing for the analysis of pathway activation in various cell types and tissues.The MAPK/ERK pathway is a crucial signaling cascade involved in regulating cell proliferation, differentiation, and survival.
Dysregulation of this pathway is often observed in cancer and other diseases, making it an attractive target for therapeutic intervention. The Phospho-ERK1/ERK2 Polyclonal Antibody provides researchers with a reliable tool to study the activity of ERK1 and ERK2 in different biological contexts, advancing our understanding of their roles in health and disease.
caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Calculated MW:
36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW:
44kDa/ 42kDa/
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
Purification Method:
Affinity purification
Gene ID:
5594/5595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (CABP0472) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
