Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Polyclonal Antibody (CABP0472)
- SKU:
- CABP0472
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- IP
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Product Name: | Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Polyclonal Antibody |
SKU: | CABP0472 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic phosphorylated peptide around T185 & Y187 of human ERK2MAPK1 (NP_002736.3). |
Sequence: | LTEY V |
Tested Applications: | WB IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 |
Synonyms: | MAPK1/MAPK3; Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 |
Positive Sample: | NIH/3T3,C6 |
Conjugate: | Unconjugated |
Cellular Localization: | caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane |
Calculated MW: | 36kDa/41kDa/38kDa/40kDa/43kDa |
Observed MW: | 44kDa/ 42kDa/ |
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
Purification Method: | Affinity purification |
Gene ID: | 5594/5595 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |