The Phospho-ERK1/2 (T202/Y204) Rabbit Polyclonal Antibody (CABP1120) is a vital tool for researchers studying the MAPK/ERK signaling pathway. Phosphorylated ERK1/2, activated at threonine 202 and tyrosine 204 residues, plays a crucial role in cell proliferation, differentiation, and response to extracellular stimuli. This antibody, developed using high-quality rabbit serum, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By targeting the phosphorylated form of ERK1/2, researchers can effectively monitor and analyze the activation status of this key signaling protein in various cell types.
The ERK1/2 pathway is involved in numerous cellular processes and is dysregulated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. Studying the phosphorylation status of ERK1/2 can provide valuable insights into disease mechanisms and potential therapeutic targets. The CABP1120 antibody enables precise detection and quantification of phospho-ERK1/2, facilitating in-depth research in cell signaling and disease pathology.
A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
Sequence:
LTEY V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Cellular Localization:
caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Observed MW:
42kDa/44kDa
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
Purification Method:
Affinity purification
Gene ID:
5594/ 5595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP1120) at 1:1000 dilution or ERK1 / ERK2 antibody (CAB16686).Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
