The Phospho-ERBB4 (Y1284) Polyclonal Antibody (CABP0034) is a valuable tool for researchers studying the ERBB4 protein, a receptor tyrosine kinase involved in cell growth and differentiation. This antibody, derived from rabbit hosts, demonstrates high specificity and sensitivity towards phosphorylated ERBB4 at tyrosine 1284 in human samples, making it ideal for Western blot applications.The phosphorylation of ERBB4 at tyrosine 1284 plays a vital role in signaling pathways related to cell proliferation, survival, and differentiation. By targeting this specific phosphorylation site, researchers can gain insights into the function and activation of ERBB4 in various cellular processes.
This antibody enables the detection and analysis of phosphorylated ERBB4 in different cell types, allowing for a deeper understanding of its role in cancer development, neuronal signaling, and other biological pathways.The Phospho-ERBB4 (Y1284) Polyclonal Antibody is a valuable tool for investigating the molecular mechanisms of ERBB4 signaling and its potential as a therapeutic target in cancer treatment and other diseases. Its high specificity and reactivity make it a reliable resource for studies in cell biology, oncology, and drug development.
A synthetic phosphorylated peptide around Y1284 of human ErbB4/HER4 (NP_005226.1).
Sequence:
PEYL S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
HER4; ALS19; p180erbB4; Phospho-ErbB4/HER4-Y1284
Positive Sample:
MCF7+IGF
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Mitochondrion, Nucleus, Single-pass type I membrane protein.
Calculated MW:
147kDa
Observed MW:
180kDa
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Purification Method:
Affinity purification
Gene ID:
2066
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells using Phospho-ErbB4/HER4-Y1284 Rabbit pAb (CABP0034) at 1:700 dilution. MCF7 cells were treated by IGF (200 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 20 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 30s.
