The Phospho-ERBB3 (Y1222) Rabbit Monoclonal Antibody (CABP1052) is a cutting-edge tool for researchers studying the phosphorylation of ERBB3 at tyrosine 1222, a critical event in cellular signaling pathways related to cell growth and survival. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity for detecting phosphorylated ERBB3 in human samples, making it an invaluable resource for studies in cancer biology and signal transduction research.ERBB3, a member of the epidermal growth factor receptor (EGFR) family, plays a key role in regulating cell proliferation and differentiation. Phosphorylation of ERBB3 at tyrosine 1222 is associated with activation of downstream signaling cascades that promote cell growth and survival, making it a focal point for investigating various cellular processes.
The Phospho-ERBB3 (Y1222) Rabbit Monoclonal Antibody enables precise detection and quantification of phosphorylated ERBB3, allowing researchers to explore the role of this protein in cancer development, progression, and resistance to therapy.By using this antibody in Western blot applications, researchers can unravel the intricate signaling networks involving phospho-ERBB3 and gain insights into potential therapeutic targets for cancer treatment. Its versatility and reliability make it an indispensable tool for unraveling the complexities of cell signaling pathways and identifying new avenues for targeted therapy.
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
148kDa
Observed MW:
185kDa
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
Purification Method:
Affinity purification
Gene ID:
2065
Clone Number:
ARC1599
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-ErbB3/HER3-Y1222 Rabbit mAb (CABP1052) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
