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Phospho-ErbB3/HER3-Y1222 Rabbit Monoclonal Antibody (CABP1052)

SKU:
CABP1052
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-ErbB3/HER3-Y1222 Rabbit Monoclonal Antibody
SKU:CABP1052
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y1222 of human ERBB3 (P21860).
Sequence:LGYE Y
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:HER3; FERLK; LCCS2; VSCN1; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; Phospho-ErbB3/HER3-Y1222
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:148kDa
Observed MW:185kDa

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

Purification Method:Affinity purification
Gene ID:2065
Clone Number:ARC1599
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of 293T cells, using Phospho-ErbB3/HER3-Y1222 Rabbit mAb (CABP1052) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.

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