The Phospho-ERBB2 (Y1221/Y1222) Monoclonal Antibody is a valuable tool for researchers studying ERBB2, a receptor tyrosine kinase involved in cell growth and survival pathways. This antibody specifically targets the phosphorylated Y1221/Y1222 sites on the ERBB2 protein, allowing for the detection and analysis of phosphorylation events in various cell types.ERBB2, also known as HER2, is a well-known oncogene often overexpressed in breast and other cancers, making it a target for cancer therapy. Phosphorylation of specific tyrosine residues, such as Y1221 and Y1222, is critical for ERBB2 activation and downstream signaling pathways.
By using this antibody in Western blot applications, researchers can gain insight into the activation status of ERBB2 in cancer cells and potentially identify novel therapeutic targets.Understanding the role of phosphorylated ERBB2 in cancer progression and response to therapy is essential for the development of precision medicine approaches. The Phospho-ERBB2 (Y1221/Y1222) Monoclonal Antibody provides researchers with a reliable tool for studying ERBB2 activation and its implications in cancer biology.
Product Name:
Phospho-ErbB2-Y1221/Y1222 Monoclonal Antibody
SKU:
CABP1424
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho synthetic peptide corresponding to residues surrounding Y1221&Y1222 of Human ErbB2.
Cell membrane, Cytoplasm, Nucleus, Nucleus, Single-pass type I membrane protein, Perinuclear region.
Calculated MW:
138kDa
Observed MW:
185kDa
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Purification Method:
Affinity purification
Gene ID:
2064
Clone Number:
ARC3121
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of A-431, using Phospho-ErbB2(Y1221 + Y1222) Rabbit mAb (CABP1424) at 1:1000 dilution.A-431 cells were treated by EGF (200 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
