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Phospho-HER2/ERBB2-Y1221/1222 Rabbit Polyclonal Antibody (CABP0527)

SKU:
CABP0527
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
$503
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Description

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Product Name:Phospho-HER2/ERBB2-Y1221/1222 Rabbit Polyclonal Antibody
SKU:CABP0527
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y1221 & Y1222 of human HER2/ERBB2 (NP_004439.2).
Sequence:NLYY WD
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; MLN-19; c-ERB2; c-ERB-2; HER-2/neu; p185(erbB2); Phospho-HER2/ErbB2-Y1221/1222
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus, Nucleus, Single-pass type I membrane protein, perinuclear region.
Calculated MW:138kDa
Observed MW:185kDa

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Purification Method:Affinity purification
Gene ID:2064
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of HeLa cells, using Phospho-HER2/ErbB2-Y1221/1222 antibody (CABP0527) at 1:1000 dilution.HeLa cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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