Phospho-EPHA3 (Tyr779/833) Antibody (PACO24109)
- SKU:
- PACO24109
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
Phospho-EPHA3 (Tyr779/833) Antibody (PACO24109)
The Phospho-EphA3 (Tyr779/833) Antibody (PAC024109) is specifically designed for research involving EphA3, a member of the Eph receptor family that plays a role in cell signaling, growth, and development. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot and immunohistochemistry applications. It specifically targets the phosphorylated forms of EphA3 at tyrosine residues 779 and 833, allowing for precise detection and analysis in a variety of cell types.EphA3 is involved in various cellular processes, including cell adhesion, migration, and angiogenesis, making it a key player in cancer progression and metastasis. The phosphorylation of EphA3 at specific sites has been linked to its activation and downstream signaling pathways, highlighting its potential as a therapeutic target in cancer research.
Understanding the role of phosphorylated EphA3 is essential for elucidating its function in tumor development and identifying new strategies for targeted cancer therapy.Overall, the Phospho-EphA3 (Tyr779/833) Antibody is a valuable tool for researchers interested in studying the signaling mechanisms of EphA3 and its implications in cancer biology. Its high specificity and sensitivity make it an ideal choice for investigations into the role of phosphorylated EphA3 in tumorigenesis and potential therapeutic interventions.
| Antibody Name: | Phospho-EPHA3 (Tyr779/833) Antibody (PACO24109) |
| Antibody SKU: | PACO24109 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:50-1:100 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Peptide sequence around phosphorylation site of tyrosine 779/833 (E-A-Y(p)-T-T)/(Q-D-V(p)-I-K) derived from Human EPHA3/4/5. |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemical analysis of paraffin-embedded human brain tissue using EPHA3/4/5 (Phospho-Tyr779/833) antibody (left)or the same antibody preincubated with blocking peptide (right). |
| Background: | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. |
| Synonyms: | EPA3; ETK; HEK; MEK4; REK |
| UniProt Protein Function: | EphA3: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family: binds to ephrin-A2, -A3, -A4 and -A5The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Two point mutations seen in a survey of colorectal tumors. Soluble receptors reduce tumor growth and angiogenesis in mouse models. Two alternatively spliced isoforms of EphA3 have been described. |
| UniProt Protein Details: | Protein type:EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; Eph family Chromosomal Location of Human Ortholog: 3p11.2 Cellular Component: early endosome; extracellular region; integral to plasma membrane; plasma membrane Molecular Function:ATP binding; GPI-linked ephrin receptor activity; protein binding Biological Process: cell adhesion; cell migration; ephrin receptor signaling pathway; peptidyl-tyrosine phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of focal adhesion formation; regulation of GTPase activity |
| NCBI Summary: | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P29320 |
| NCBI GenInfo Identifier: | 116241351 |
| NCBI Gene ID: | 2042 |
| NCBI Accession: | P29320.2 |
| UniProt Secondary Accession: | P29320,Q9H2V3, Q9H2V4, |
| UniProt Related Accession: | P29320 |
| Molecular Weight: | 60,946 Da |
| NCBI Full Name: | Ephrin type-A receptor 3 |
| NCBI Synonym Full Names: | EPH receptor A3 |
| NCBI Official Symbol: | EPHA3Â Â |
| NCBI Official Synonym Symbols: | EK4; ETK; HEK; ETK1; HEK4; TYRO4Â Â |
| NCBI Protein Information: | ephrin type-A receptor 3 |
| UniProt Protein Name: | Ephrin type-A receptor 3 |
| UniProt Synonym Protein Names: | EPH-like kinase 4; EK4; hEK4; HEK; Human embryo kinase; Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1; Eph-like tyrosine kinase 1 |
| Protein Family: | Ephrin type-A receptor |
| UniProt Gene Name: | EPHA3Â Â |
| UniProt Entry Name: | EPHA3_HUMAN |
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