The Phospho-EphA2 (Y594) Polyclonal Antibody (CABP0818) is a valuable tool for researchers studying EphA2, a member of the Eph receptor family involved in various cellular processes such as cell adhesion, migration, and angiogenesis. This antibody, generated in rabbits, is highly specific to the phosphorylated form of EphA2 at Tyr594 and is validated for use in Western blot analysis.EphA2 signaling has been implicated in cancer progression, making it a potential target for cancer therapy. Phosphorylation of EphA2 at Tyr594 has been linked to increased oncogenic potential, highlighting the importance of studying this specific site in cancer research. The Phospho-EphA2 (Y594) Polyclonal Antibody allows for the detection and analysis of phosphorylated EphA2 in various cell types, providing valuable insights into its role in tumorigenesis and metastasis.
Researchers interested in understanding the molecular mechanisms underlying EphA2 signaling and its implications in cancer biology will find the Phospho-EphA2 (Y594) Polyclonal Antibody to be a reliable and specific tool for their studies. Its ability to specifically recognize the phosphorylated form of EphA2 at Tyr594 makes it a valuable resource for investigating the role of EphA2 in cancer progression and developing targeted therapies against this pathway.
Product Name:
Phospho-EphA2-Y594 Rabbit Polyclonal Antibody
SKU:
CABP0818
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around Y594 of human EphA2 (NP_004422.2).
Cell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, focal adhesion, lamellipodium membrane, ruffle membrane.
Calculated MW:
108kDa
Observed MW:
108kDa
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.
Purification Method:
Affinity purification
Gene ID:
1969
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from SH-SY5Y cells, using Phospho-EPHA2-Y594 Rabbit pAb (CABP0818) at 1:1000 dilution. SH-SY5Y cells were treated by EphrinA1 (100 ng/mL) for 5 minutes .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
