The Phospho-EPHA2 (S897) Rabbit Polyclonal Antibody (CABP1082) is a valuable tool for researchers studying the phosphorylation of EPHA2, a receptor tyrosine kinase involved in various cellular processes, including cell adhesion, migration, and proliferation. This antibody, raised in rabbits, exhibits high reactivity with samples from a variety of species and is validated for use in Western blot applications.EPHA2 phosphorylation at the S897 site has been linked to the regulation of cell signaling pathways implicated in cancer progression, making it a key target for investigation in cancer research. The antibody specifically recognizes the phosphorylated form of EPHA2 at S897, enabling precise detection and quantification of this critical modification in cell lysates.
Its reliable performance makes it suitable for studies focusing on signaling pathways, cell biology, and cancer biology. By using the Phospho-EPHA2 (S897) Rabbit Polyclonal Antibody (CABP1082), researchers can gain valuable insights into the role of EPHA2 phosphorylation in cancer development and potentially identify new therapeutic targets for various types of cancer. This antibody is an essential tool for advancing our understanding of the molecular mechanisms underlying cancer progression and may contribute to the development of targeted therapies for cancer treatment.
Product Name:
Phospho-EPHA2-S897 Rabbit Polyclonal Antibody
SKU:
CABP1082
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S897 of human EPHA2 (NP_004422.2).
Cell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, focal adhesion, lamellipodium membrane, ruffle membrane.
Calculated MW:
108kDa
Observed MW:
125kDa
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.
Purification Method:
Affinity purification
Gene ID:
1969
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of MDA-MB-231, using Phospho-EPHA2-S897 Rabbit pAb (CABP1082) at 1:400 dilution.MDA-MB-231 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
