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Phospho-EPHA2/EPHA3/EPHA4 (Tyr588/596) Antibody (PACO24381)

SKU:
PACO24381
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • Phospho-EPHA2/EPHA3/EPHA4 Tyr588/596 Antibody PACO24381
  • Phospho-EPHA2/EPHA3/EPHA4 Tyr588/596 Antibody PACO24381
€549
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Description

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Phospho-EPHA2/EPHA3/EPHA4 (Tyr588/596) Antibody (PACO24381)

The Phospho-EphA2/EphA3/EphA4 (Tyr588/596) Antibody (PAC024381) is a highly specific tool for research involving the phosphorylation of Eph receptors, particularly EphA2, EphA3, and EphA4, at tyrosine residues 588 and 596. This antibody, raised in rabbits, is suitable for various applications such as Western blot and immunoprecipitation assays and is validated for use with human samples.Eph receptors are a family of receptor tyrosine kinases involved in numerous biological processes, including cell adhesion, migration, and axon guidance. Phosphorylation of Eph receptors, such as EphA2, EphA3, and EphA4, at specific tyrosine residues, like 588 and 596, is known to regulate their activity and signaling pathways.

Research into the phosphorylation status of these receptors is essential for understanding their role in development, cancer progression, and neurological diseases.This Phospho-EphA2/EphA3/EphA4 (Tyr588/596) Antibody (PAC024381) offers researchers a reliable tool to study the phosphorylation status of Eph receptors in various biological contexts. Its specificity and sensitivity make it a valuable asset for studies in cell signaling, cancer biology, and neuroscience, providing insights into the molecular mechanisms underlying Eph receptor function and potential therapeutic targeting opportunities.

Antibody Name:Phospho-EPHA2/EPHA3/EPHA4 (Tyr588/596) Antibody (PACO24381)
Antibody SKU:PACO24381
Size:100ul
Host Species:Rabbit
Tested Applications:ELISA, WB, IF
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:1000, IF:1:100-1:200
Species Reactivity:Human, Mouse
Immunogen:Peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y(p)-V-R)/(R-T-Y(p)-V-D) derived from Human EPHA2/3/4.
Form:Liquid
Storage Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody.The lane on the right is treated with the antigen-specific peptide.
Product ImageImmunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody.
Background:

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

Synonyms:ECK; MPK-5; SEK2; kinase EphA2;
UniProt Protein Function:EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice.
UniProt Protein Details:

Protein type:Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cell surface; focal adhesion; integral to plasma membrane; intracellular; plasma membrane

Molecular Function:ATP binding; ephrin receptor activity; protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: angiogenesis; axial mesoderm formation; axon guidance; bone remodeling; cell adhesion; cell migration; DNA damage response, signal transduction resulting in induction of apoptosis; ephrin receptor signaling pathway; keratinocyte differentiation; mammary gland epithelial cell proliferation; multicellular organismal development; negative regulation of protein kinase B signaling cascade; neural tube development; notochord cell development; notochord formation; osteoblast differentiation; osteoclast differentiation; peptidyl-tyrosine phosphorylation; protein kinase B signaling cascade; regulation of angiogenesis; regulation of blood vessel endothelial cell migration; regulation of cell adhesion mediated by integrin; skeletal development; vasculogenesis; viral reproduction

Disease: Cataract 6, Multiple Types

NCBI Summary:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]
UniProt Code:P29317
NCBI GenInfo Identifier:229462861
NCBI Gene ID:1969
NCBI Accession:P29317.2
UniProt Secondary Accession:P29317,Q8N3Z2, B5A968,
UniProt Related Accession:P29317
Molecular Weight:
NCBI Full Name:Ephrin type-A receptor 2
NCBI Synonym Full Names:EPH receptor A2
NCBI Official Symbol:EPHA2  
NCBI Official Synonym Symbols:ECK; CTPA; ARCC2; CTPP1; CTRCT6  
NCBI Protein Information:ephrin type-A receptor 2
UniProt Protein Name:Ephrin type-A receptor 2
UniProt Synonym Protein Names:Epithelial cell kinase; Tyrosine-protein kinase receptor ECK
Protein Family:Ephrin type-A receptor
UniProt Gene Name:EPHA2  
UniProt Entry Name:EPHA2_HUMAN
AntibodiesELISA Kits
Phospho-EPHA2/EPHA3/EPHA4 (Y588/596) Antibody (PACO02630)EPHA2/3/4 Colorimetric Cell-Based ELISA Kit
EPHA2/EPHA3/EPHA4 Antibody (PACO06464)Phospho-EPHA2/3/4 (Tyr588/596) In-Cell ELISA
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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