The Phospho-eNOS (T495) Polyclonal Antibody (CABP0516) is a valuable tool for researchers studying eNOS, an essential enzyme involved in the production of nitric oxide and regulation of vascular tone. This antibody is raised in rabbits and specifically detects phosphorylation of eNOS at threonine 495, a post-translational modification that is important for eNOS activity.This reliable antibody is highly reactive with human samples and has been validated for use in Western blot applications, allowing for precise and sensitive detection of phosphorylated eNOS in various cell types and tissues. Its ability to specifically target the phosphorylated form of eNOS makes it an essential tool for investigating the role of eNOS phosphorylation in physiological and pathological processes, such as cardiovascular disease, diabetes, and neurodegenerative disorders.
By enabling researchers to study the phosphorylation status of eNOS at threonine 495, the Phospho-eNOS (T495) Polyclonal Antibody (CABP0516) provides valuable insights into the regulation of eNOS activity and its impact on vascular function, making it an indispensable tool for studies in cardiovascular biology, pharmacology, and drug development.
Product Name:
Phospho-eNOS-T495 Rabbit Polyclonal Antibody
SKU:
CABP0516
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T495 of human eNOS (NP_000594.2).
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4846
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eNOS-T495 antibody (CABP0516) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
