Anti-Phospho-eNOS-S1177 Antibody (CABP0515)
- SKU:
- CABP0515
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-Phospho-eNOS-S1177 Antibody (CABP0515)
The Phospho-eNOS (S1177) Polyclonal Antibody (CABP0515) is a vital tool for researchers studying phosphorylation of the endothelial nitric oxide synthase (eNOS) at the Ser1177 residue. This antibody, generated in rabbits, exhibits high reactivity with human and mouse samples, and has been validated for use in Western blot and immunohistochemistry applications.eNOS is a crucial enzyme involved in the production of nitric oxide, a key signaling molecule in the cardiovascular system. Phosphorylation of eNOS at Ser1177 is known to enhance its enzymatic activity, leading to increased nitric oxide production and vasodilation.
Dysregulation of eNOS phosphorylation has been linked to various cardiovascular diseases, including hypertension and atherosclerosis.By using the Phospho-eNOS (S1177) Polyclonal Antibody, researchers can investigate the impact of phosphorylation on eNOS function in different cell types and pathological conditions. This antibody is an essential tool for advancing our understanding of nitric oxide signaling in cardiovascular physiology and pathology, with potential implications for the development of novel therapeutic strategies for cardiovascular disorders.
| Antibody Name: | Anti-Phospho-eNOS-S1177 Antibody |
| Antibody SKU: | CABP0515 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SKOV3, NIH/3T3 |
| Immunogen: | A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TQSF S |
| Gene ID: | 4846 |
| Uniprot: | P29474 |
| Cellular Location: | Cell membrane, Cytoplasm, Golgi apparatus, Membrane, caveola, cytoskeleton |
| Calculated MW: | 67kDa/68kDa/133kDa |
| Observed MW: | 133kDa |
 | Western blot analysis of extracts of SKOV3 cells, using Phospho-eNOS-S1177 antibody (CABP0515). SKOV3 cells were treated by EGF. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eNOS-S1177 antibody (CABP0515) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using Phospho-NOS3-S1177 antibody (CABP0515) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-NOS3-S1177 antibody (CABP0515) at dilution of 1:200 (40x lens). |
| Synonyms: | NOS3, ECNOS, eNOS |
| Background: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
| UniProt Protein Function: | eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39 Chromosomal Location of Human Ortholog: 7q36 Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; Golgi membrane; nucleus; plasma membrane Molecular Function:actin monomer binding; cadmium ion binding; calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding Biological Process: angiogenesis; arginine catabolic process; blood coagulation; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; mitochondrion organization and biogenesis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of potassium ion transport; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; nitric oxide metabolic process; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood pressure; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to heat; smooth muscle hyperplasia; vascular endothelial growth factor receptor signaling pathway Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic |
| NCBI Summary: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| UniProt Code: | P29474 |
| NCBI GenInfo Identifier: | 266648 |
| NCBI Gene ID: | 4846 |
| NCBI Accession: | P29474.3 |
| UniProt Secondary Accession: | P29474,Q13662, Q14251, Q14434, Q548C1, Q6GSL5, Q9UDC6 A0S0A7, A0S0A8, A8KA63, B2RCQ1, E9PFR2, |
| UniProt Related Accession: | P29474 |
| Molecular Weight: | |
| NCBI Full Name: | Nitric oxide synthase, endothelial |
| NCBI Synonym Full Names: | nitric oxide synthase 3 |
| NCBI Official Symbol: | NOS3Â Â |
| NCBI Official Synonym Symbols: | eNOS; ECNOSÂ Â |
| NCBI Protein Information: | nitric oxide synthase, endothelial |
| UniProt Protein Name: | Nitric oxide synthase, endothelial |
| UniProt Synonym Protein Names: | Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII |
| Protein Family: | Nitric oxide synthase |
| UniProt Gene Name: | NOS3Â Â |
| UniProt Entry Name: | NOS3_HUMAN |
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