The Phospho-eNOS (S1177) Monoclonal Antibody is a valuable tool for researchers studying endothelial nitric oxide synthase (eNOS) activation. This antibody, produced using monoclonal technology, specifically recognizes the phosphorylated form of eNOS at serine 1177, a key regulatory site for eNOS activation. eNOS is an important enzyme involved in the production of nitric oxide, a signaling molecule that plays a critical role in regulating blood flow, vascular tone, and cardiovascular health. Phosphorylation of eNOS at S1177 is known to enhance its activity, leading to increased nitric oxide production and vasodilation.
This antibody is highly specific for detecting phosphorylated eNOS at S1177 in human samples, making it ideal for use in Western blot and immunofluorescence applications. By studying eNOS activation and function, researchers can gain insights into cardiovascular diseases, endothelial dysfunction, and other conditions where nitric oxide signaling is dysregulated.Overall, the Phospho-eNOS (S1177) Monoclonal Antibody offers a powerful tool for investigating the role of eNOS activation in health and disease, paving the way for potential therapeutic interventions targeting nitric oxide signaling pathways.
Product Name:
Phospho-eNOS-S1177 Monoclonal Antibody
SKU:
CABP1404
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4846
Clone Number:
ARC58017
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of 293T lysates, using Phospho-eNOS-S1177 antibody (CABP1404) at 1:7000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
