The Phospho-eIF4EBP1 (T70) Monoclonal Antibody is a crucial tool for researchers studying the phosphorylation of eIF4EBP1 at threonine 70, a key event in the regulation of protein synthesis and cell growth. This antibody, generated through a rigorous process using hybridoma technology, specifically recognizes the phosphorylated form of eIF4EBP1 and is validated for use in various experimental techniques, including Western blotting and immunohistochemistry.eIF4EBP1 is a key regulator of protein translation and is often dysregulated in cancer and other diseases characterized by abnormal cell growth. Phosphorylation of eIF4EBP1 at T70 is known to enhance its inhibitory effect on translation initiation, making it a critical target for research into mechanisms of tumorigenesis and potential therapeutic interventions.
The specificity and sensitivity of this monoclonal antibody make it an essential tool for dissecting the signaling pathways involved in eIF4EBP1 phosphorylation and its downstream effects.By utilizing the Phospho-eIF4EBP1 (T70) Monoclonal Antibody, researchers can gain valuable insights into the intricate regulatory mechanisms governing protein synthesis and cell proliferation, paving the way for innovative approaches to cancer therapy and other disease treatments.
Product Name:
Phospho-eIF4EBP1-T70 Monoclonal Antibody
SKU:
CABP1334
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around T70 of human eIF4EBP1 (NP_004086.1).
Sequence:
TKTP PR
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Synonyms:
BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T70
Positive Sample:
293T+FBS,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Cytosol
Calculated MW:
13kDa
Observed MW:
15-20kDa
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method:
Affinity purification
Gene ID:
1978
Clone Number:
ARC53785
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-eIF4EBP1-T70 Rabbit mAb (CABP1334) at 1:2000 dilution.293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
