The Phospho-eIF4EBP1 (T37) Monoclonal Antibody is a cutting-edge tool for researchers studying the eIF4EBP1 protein, a key regulator of protein synthesis and cell growth. This antibody, developed and validated by Assay Genie, specifically targets the phosphorylated form of eIF4EBP1 at threonine 37, offering high specificity and sensitivity in Western blot and immunohistochemistry applications.eIF4EBP1 is a crucial player in the mTOR signaling pathway, controlling translation initiation and cell proliferation. Phosphorylation of eIF4EBP1 at T37 is known to regulate its activity and downstream effects on protein synthesis.
By using this monoclonal antibody, researchers can accurately monitor the phosphorylation status of eIF4EBP1 in various cell types and experimental conditions, providing valuable insights into its role in cancer, metabolic diseases, and neurological disorders.With its superior performance and reliability, the Phospho-eIF4EBP1 (T37) Monoclonal Antibody from Assay Genie is a must-have for scientists seeking to unravel the complex mechanisms of protein synthesis regulation and its implications in health and disease.
Product Name:
Phospho-eIF4EBP1-T37 Monoclonal Antibody
SKU:
CABP1382
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic phosphorylated peptide around T37 of human EIF4EBP1.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T37
Positive Sample:
C6
Conjugate:
Unconjugated
Calculated MW:
13kDa
Observed MW:
15-20kDa
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method:
Affinity purification
Gene ID:
1978
Clone Number:
ARC56857
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts from C6 cells, using Phospho-eIF4EBP1-T37 Rabbit mAb (CABP1382) at 1:1000 dilution.C6 cells were treated by IGF-1 (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
