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Phospho-eIF4EBP1-T37 Monoclonal Antibody (CABP1382)

SKU:
CABP1382
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €609
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-eIF4EBP1-T37 Monoclonal Antibody
SKU:CABP1382
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Rat
Immunogen:A synthetic phosphorylated peptide around T37 of human EIF4EBP1.
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T37
Positive Sample:C6
Conjugate:Unconjugated
Calculated MW:13kDa
Observed MW:15-20kDa

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Purification Method:Affinity purification
Gene ID:1978
Clone Number:ARC56857
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts from C6 cells, using Phospho-eIF4EBP1-T37 Rabbit mAb (CABP1382) at 1:1000 dilution.C6 cells were treated by IGF-1 (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.

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