The Phospho-eIF4EBP1 (T37/46) Polyclonal Antibody (CABP0030) is a valuable tool for researchers studying eIF4EBP1, a key protein involved in the regulation of protein synthesis and cell growth. This antibody, produced in rabbits, specifically targets the phosphorylated form of eIF4EBP1 at threonine residues 37 and 46, allowing for precise detection and analysis of this important modification.Phosphorylation of eIF4EBP1 at T37/46 is known to regulate its interaction with eukaryotic translation initiation factor 4E (eIF4E), ultimately influencing protein translation and cell proliferation. Understanding the dynamics of eIF4EBP1 phosphorylation is crucial in elucidating its role in various cellular processes, including cell cycle progression, differentiation, and survival.
Validated for use in Western blot applications, the Phospho-eIF4EBP1 (T37/46) Polyclonal Antibody is highly specific for human samples, making it an ideal tool for researchers investigating the role of eIF4EBP1 phosphorylation in diseases such as cancer, neurodegenerative disorders, and metabolic diseases. By targeting this specific phosphorylation event, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method:
Affinity purification
Gene ID:
1978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-eIF4EBP1-T37/46 Rabbit pAb (CABP0030) at 1:1000 dilution.HeLa cells were treated by IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
