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Phospho-eIF4EBP1-T37/46 Rabbit Polyclonal Antibody (CABP0030)

SKU:
CABP0030
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-eIF4EBP1-T37/46 Rabbit Polyclonal Antibody
SKU:CABP0030
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T37 & T46 of human eIF4EBP1 (NP_004086.1).
Sequence:STTP GGTL FSTT PG
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T37/46
Positive Sample:HeLa,NIH/3T3,C6
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol
Calculated MW:13kDa
Observed MW:15kDa-20kDa

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Purification Method:Affinity purification
Gene ID:1978
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-eIF4EBP1-T37/46 Rabbit pAb (CABP0030) at 1:1000 dilution.HeLa cells were treated by IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.

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