The Phospho-eIF4EBP1 (T36) Polyclonal Antibody (CABP0344) is a valuable tool for researchers studying eIF4EBP1, a key protein involved in the regulation of protein translation. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards phospho-eIF4EBP1 at threonine 36, making it an ideal choice for Western blotting and immunohistochemistry applications.eIF4EBP1 is a critical player in the mTOR signaling pathway, which regulates protein synthesis in response to various cellular signals. Phosphorylation of eIF4EBP1 at threonine 36 is known to modulate its activity, impacting protein translation and cell growth.
Research into the function of phosphorylated eIF4EBP1 is crucial for understanding its role in diseases such as cancer, where dysregulation of protein synthesis can contribute to tumor development and progression.By utilizing the Phospho-eIF4EBP1 (T36) Polyclonal Antibody, researchers can investigate the signaling pathways and molecular mechanisms involving eIF4EBP1 phosphorylation, providing valuable insights into disease pathology and potential therapeutic targets. This antibody offers a reliable tool for studying the regulation of protein translation and its implications in cellular processes and diseases.
Product Name:
Phospho-eIF4EBP1-T36 Rabbit Polyclonal Antibody
SKU:
CABP0344
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T36 of human eIF4EBP1 (NP_004086.1).
Sequence:
YSTT P
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T36
Positive Sample:
MDA-MB-435
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol
Calculated MW:
13kDa
Observed MW:
20kDa
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method:
Affinity purification
Gene ID:
1978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from MDA-MB-435 cells, using Phospho-eIF4EBP1-T36 Rabbit pAb (CABP0344).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
