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Phospho-eIF4EBP1-S65 Rabbit Polyclonal Antibody (CABP0032)

SKU:
CABP0032
Product Type:
Antibody
Applications:
WB
IP
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-eIF4EBP1-S65 Rabbit Polyclonal Antibody
SKU:CABP0032
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S65 of human Phospho-eIF4EBP1-S65 (NP_004086.1).
Sequence:RNSP V
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-S65
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol
Calculated MW:13kDa
Observed MW:15-20kDa

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Purification Method:Affinity purification
Gene ID:1978
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from 293T cells using Phospho-eIF4EBP1-S65 Rabbit pAb (CABP0032) at 1:700 dilution. 293T cells were treated by λPP mixed solution (1ul) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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