The Phospho-eIF4EBP1 (S65) Polyclonal Antibody (CABP0032) is a valuable tool for research involving the phosphorylation of eIF4EBP1 at Serine 65, a key regulator of mRNA translation initiation. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. It specifically recognizes the phosphorylated form of eIF4EBP1 at Serine 65, allowing for precise detection and analysis in various cell types.eIF4EBP1 is a crucial protein involved in the control of protein synthesis, particularly in response to cellular stress and growth signaling pathways. Phosphorylation of eIF4EBP1 at Serine 65 is known to regulate its activity, ultimately impacting global protein translation.
Understanding the mechanisms behind eIF4EBP1 phosphorylation is essential for unraveling its role in cellular processes and disease states.Researchers studying signaling pathways related to protein synthesis, cellular stress responses, and cancer biology will find the Phospho-eIF4EBP1 (S65) Polyclonal Antibody to be a valuable asset in their experiments. Its specificity for the phosphorylated form of eIF4EBP1 at Serine 65 makes it an excellent tool for investigating the intricate regulation of mRNA translation initiation in various biological contexts.
Product Name:
Phospho-eIF4EBP1-S65 Rabbit Polyclonal Antibody
SKU:
CABP0032
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S65 of human Phospho-eIF4EBP1-S65 (NP_004086.1).
Sequence:
RNSP V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-S65
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol
Calculated MW:
13kDa
Observed MW:
15-20kDa
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method:
Affinity purification
Gene ID:
1978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells using Phospho-eIF4EBP1-S65 Rabbit pAb (CABP0032) at 1:700 dilution. 293T cells were treated by λPP mixed solution (1ul) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
