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Phospho-eIF4EBP1-S65 Monoclonal Antibody (CABP1363)

SKU:
CABP1363
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $731
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-eIF4EBP1-S65 Monoclonal Antibody
SKU:CABP1363
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around S65 of human Phospho-EIF4EBP1-S65 (NP_004086.1).
Sequence:RNSP V
Tested Applications:WB ELISA
Recommended Dilution:WB,1:2000 - 1:20000
Synonyms:BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-S65
Positive Sample:293T,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol
Calculated MW:13kDa
Observed MW:15-20kDa

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Purification Method:Affinity purification
Gene ID:1978
Clone Number:ARC53775
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of 293T, using Phospho-EIF4EBP1-S65 antibody (CABP1363) at 1:20000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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