The Phospho-EIF4EBP1 (S65) Monoclonal Antibody (CABP1363) is a powerful tool for research involving EIF4EBP1, a protein involved in the regulation of protein synthesis. This antibody, derived from mouse cells, specifically targets the phosphorylated form of EIF4EBP1 at serine 65, allowing for precise detection and analysis in various experimental settings.Phosphorylation of EIF4EBP1 at serine 65 is known to play a crucial role in the regulation of protein translation, making this antibody essential for studying cellular processes such as cell growth, proliferation, and survival.
Its high specificity for the phosphorylated form of EIF4EBP1 ensures accurate results in techniques like Western blotting and immunofluorescence.Researchers in the fields of molecular biology, cell signaling, and cancer biology will find this antibody invaluable for investigating the role of EIF4EBP1 in various cellular pathways and disease processes. By targeting the phosphorylated form of EIF4EBP1, this antibody opens up new possibilities for understanding the intricate mechanisms governing protein synthesis and cellular function.
Product Name:
Phospho-eIF4EBP1-S65 Monoclonal Antibody
SKU:
CABP1363
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S65 of human Phospho-EIF4EBP1-S65 (NP_004086.1).
Sequence:
RNSP V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:2000 - 1:20000
Synonyms:
BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-S65
Positive Sample:
293T,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol
Calculated MW:
13kDa
Observed MW:
15-20kDa
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method:
Affinity purification
Gene ID:
1978
Clone Number:
ARC53775
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of 293T, using Phospho-EIF4EBP1-S65 antibody (CABP1363) at 1:20000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
