The Phospho-eIF4E (S209) Rabbit Monoclonal Antibody is a valuable tool for researchers studying the phosphorylation of eIF4E, a key protein involved in the initiation of translation. This antibody, produced in rabbits, is highly specific for the phosphorylated form of eIF4E at serine 209 and is validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can investigate the regulation of eIF4E activity in various cellular processes, such as protein synthesis, cell growth, and proliferation.The phosphorylation of eIF4E at serine 209 is known to regulate its function in cap-dependent translation, a process essential for the synthesis of proteins involved in cell proliferation and survival. Aberrant regulation of eIF4E phosphorylation has been implicated in various diseases, including cancer, where dysregulated protein synthesis can promote tumor growth and progression.
By studying the phosphorylation of eIF4E at serine 209, researchers can gain insights into the role of this critical translational regulator in disease pathogenesis and potentially identify new therapeutic targets for intervention.Overall, the Phospho-eIF4E (S209) Rabbit Monoclonal Antibody offers researchers a reliable tool for investigating the phosphorylation status of eIF4E and its impact on cellular processes and disease development. Its specificity and sensitivity make it an essential component for studies in cancer biology, molecular biology, and translational research.
Product Name:
Phospho-eIF4E-S209 Monoclonal Antibody
SKU:
CABP1024
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding S209 of human Phospho-eIF4E-S209 (NP_001959.1).
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1977
Clone Number:
ARC1569
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-eIF4E-S209 antibody (CABP1024) at 1:1000 dilution.HeLa cells were treated by 20% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
