The Phospho-eIF4B (S422) Polyclonal Antibody (CABP0775) is a valuable tool for researchers studying the phosphorylation of eIF4B at serine 422. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to phosphorylated eIF4B at S422, this antibody enables the detection and analysis of this important protein modification in various biological samples.eIF4B is a key regulator of protein synthesis and plays a vital role in the translation of specific mRNAs. Phosphorylation of eIF4B at S422 has been implicated in the regulation of mRNA translation, and dysregulation of this process has been associated with various diseases, including cancer and neurological disorders.
The Phospho-eIF4B (S422) Polyclonal Antibody provides researchers with a reliable tool to investigate the role of eIF4B phosphorylation in cellular processes and disease mechanisms.By understanding the mechanisms underlying eIF4B phosphorylation at S422, researchers can gain insights into the regulation of protein synthesis and potentially develop new therapeutic strategies targeting this pathway. The Phospho-eIF4B (S422) Polyclonal Antibody is a valuable resource for studies in molecular biology, cancer research, and other fields where protein translation plays a critical role.
Product Name:
Phospho-EIF4B-S422 Rabbit Polyclonal Antibody
SKU:
CABP0775
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S422 of human EIF4B (NP_001408.2).
Enables RNA binding activity. Predicted to be involved in eukaryotic translation initiation factor 4F complex assembly and formation of translation preinitiation complex. Located in cytosol. Biomarker of autism spectrum disorder and major depressive disorder.
Purification Method:
Affinity purification
Gene ID:
1975
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-EIF4B-S422 Rabbit pAb (CABP0775) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
