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eIF2α Rabbit Polyclonal Antibody (CAB0764)

SKU:
CAB0764
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 1 Anti-eIF2Alpha Antibody CAB0764
  • Metabolism Antibodies 1 Anti-eIF2Alpha Antibody CAB0764
  • Metabolism Antibodies 1 Anti-eIF2Alpha Antibody CAB0764
  • Metabolism Antibodies 1 Anti-eIF2Alpha Antibody CAB0764
  • Metabolism Antibodies 1 Anti-eIF2Alpha Antibody CAB0764
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:eIF2α Rabbit Polyclonal Antibody
SKU:CAB0764
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human eIF2α (NP_004085.1).
Sequence:MPGL SCRF YQHK FPEV EDVV MVNV RSIA EMGA YVSL LEYN NIEG MILL SELS RRRI RSIN KLIR IGRN ECVV VIRV DKEK GYID LSKR RVSP EEAI KCED KFTK SKTV YSIL RHVA EVLE YTKD EQLE SLFQ RTAW VFDD KYKR PGYG AYDA FKHA VSDP SILD SLDL NEDE REVL INNI NRRL TPQA VKIR ADIE VACY GYEG IDAV KEAL RAGL NCST ENMP IKIN LIAP PRYV MTTT TLER TEGL SVLS QAMA VIKE
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF2α
Positive Sample:HeLa,MCF7,293F,NIH/3T3,Mouse liver,Rat testis,PC-12
Conjugate:Unconjugated
Cellular Localization:Cytoplasmic granule.
Calculated MW:36kDa
Observed MW:38kDa

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Purification Method:Affinity purification
Gene ID:1965
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using eIF2α antibody (CAB0764) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

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