Anti-Phospho-eIF2Alpha-S51 Antibody (CABP0692)
- SKU:
- CABP0692
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Metabolism
Description
Anti-Phospho-eIF2Alpha-S51 Antibody (CABP0692)
The Phospho-eIF2alpha (S51) Rabbit Monoclonal Antibody (CABP0692) is a valuable tool for researchers studying the phosphorylation of eIF2alpha at serine 51, a key event in the regulation of protein synthesis and cellular stress responses. This antibody, derived from rabbit monoclonal cells, exhibits high specificity and sensitivity for detecting phosphorylated eIF2alpha in human samples.Phosphorylation of eIF2alpha at serine 51 is a critical regulator of protein translation, controlling the synthesis of stress-response proteins in various cellular processes such as apoptosis, autophagy, and unfolded protein response. Dysregulation of eIF2alpha phosphorylation has been implicated in a range of diseases, including neurodegenerative disorders, viral infections, and cancer.
The Phospho-eIF2alpha (S51) Rabbit Monoclonal Antibody enables precise detection and quantification of phosphorylated eIF2alpha levels in cell lysates and tissue samples, making it an essential tool for investigations into the molecular mechanisms underlying cellular stress responses and disease pathogenesis. Researchers in fields such as cell biology, molecular biology, and cancer research will find this antibody invaluable for their studies of eIF2alpha phosphorylation dynamics.
| Antibody Name: | Anti-Phospho-eIF2Alpha-S51 Antibody |
| Antibody SKU: | CABP0692 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human Phospho-EIF2S1 (S51). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3 |
| Immunogen: | A synthesized peptide derived from human Phospho-EIF2S1 (S51). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 1965 |
| Uniprot: | P05198 |
| Cellular Location: | |
| Calculated MW: | 36kDa |
| Observed MW: | 36KDa |
 | Western blot - Phospho-eIF2Alpha-S51 Rabbit mAb (CABP0692) |
| Synonyms: | EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, EIF2S1 |
| Background: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| UniProt Protein Function: | eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections. |
| UniProt Protein Details: | Protein type:RNA-binding; Translation initiation; Translation Chromosomal Location of Human Ortholog: 14q23.3 Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex Molecular Function:protein binding; translation initiation factor activity; ribosome binding Biological Process: regulation of translation initiation in response to stress; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression |
| NCBI Summary: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| UniProt Code: | P05198 |
| NCBI GenInfo Identifier: | 124200 |
| NCBI Gene ID: | 1965 |
| NCBI Accession: | P05198.3 |
| UniProt Related Accession: | P05198 |
| Molecular Weight: | 315 |
| NCBI Full Name: | Eukaryotic translation initiation factor 2 subunit 1 |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa |
| NCBI Official Symbol: | EIF2S1Â Â |
| NCBI Official Synonym Symbols: | EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha  |
| NCBI Protein Information: | eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha |
| UniProt Protein Name: | Eukaryotic translation initiation factor 2 subunit 1 |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF2S1Â Â |
| UniProt Entry Name: | IF2A_HUMAN |
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