The Phospho EIF2AK2 (T446) Rabbit Monoclonal Antibody (CABP1134) is a valuable tool for researchers studying EIF2AK2, a protein involved in the regulation of immune responses and cellular stress. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. With a high level of reactivity, this antibody allows for the precise detection and analysis of EIF2AK2 in a variety of cell types, making it an ideal choice for studies in immunology and cancer research.EIF2AK2, also known as protein kinase R (PKR), is a key player in immune response regulation and is important for maintaining cellular homeostasis in response to stress.
It is involved in the control of viral infections, as well as in the regulation of cell growth and survival. Understanding the role of EIF2AK2 in these processes is essential for developing targeted therapies for diseases such as cancer, viral infections, and autoimmune disorders. The Phospho EIF2AK2 (T446) Rabbit Monoclonal Antibody (CABP1134) provides researchers with a powerful tool for investigating the function of EIF2AK2 and its potential implications in various disease contexts.
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.
Purification Method:
Affinity purification
Gene ID:
5610
Clone Number:
ARC0293
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-PKR/EIF2AK2-T446 Rabbit mAb (CAB4869) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
