The PKR Rabbit Monoclonal Antibody (CAB19545) is a vital tool for research involving PKR, also known as Protein Kinase R, a key player in the immune response and antiviral defense. This monoclonal antibody, derived from rabbit sources, is highly specific and sensitive for detecting PKR in various experimental settings.PKR is a serine/threonine protein kinase that is activated in response to viral infection, leading to the inhibition of protein synthesis and the induction of apoptosis in infected cells. By targeting PKR with this antibody, researchers can study its role in antiviral defense mechanisms, as well as its involvement in other cellular processes such as inflammation and stress response.
This antibody is validated for use in techniques such as Western blotting, immunofluorescence, and immunohistochemistry, making it a versatile tool for studying PKR expression and activity in different cell types and tissues. Its high specificity and sensitivity ensure reliable and reproducible results, making it an essential reagent for studies in virology, immunology, and cellular signaling pathways.
Product Name:
PKR/EIF2AK2 Rabbit Monoclonal Antibody
SKU:
CAB19545
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 450-551 of human PKR/EIF2AK2 (P19525).
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.
Purification Method:
Affinity purification
Gene ID:
5610
Clone Number:
ARC0024
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PKR/EIF2AK2 antibody (CAB19545) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
