The Phospho-EGFR (Y992) Polyclonal Antibody (CABP0026) is a valuable tool for researching the phosphorylation of the EGFR protein at tyrosine 992, a key site involved in signal transduction and cellular proliferation. The antibody, produced in rabbits, has high specificity for human samples and is suitable for use in Western blotting and immunohistochemistry applications.EGFR, or epidermal growth factor receptor, plays a crucial role in cell growth, survival, and differentiation, making it a significant target for cancer research and drug development.
Phosphorylation of EGFR at tyrosine 992 has been linked to various cancer types, providing insights into tumor development and potential therapeutic strategies.Studying the phosphorylation status of EGFR at specific sites, such as Y992, can provide valuable information on cell signaling pathways and potential drug targets in cancer therapy. The Phospho-EGFR (Y992) Polyclonal Antibody is an essential tool for researchers investigating EGFR signaling and its implications in cancer biology.
Product Name:
Phospho-EGFR-Y992 Rabbit Polyclonal Antibody
SKU:
CABP0026
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y992 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
180kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y992 antibody (CABP0026) at 1:1000 dilution. A431 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
