The Phospho-EGFR (Y845) Polyclonal Antibody (CABP0023) is a powerful tool for studying phosphorylated Epidermal Growth Factor Receptor (EGFR) at tyrosine 845 residue. EGFR is a key player in cell growth, proliferation, and survival, and its phosphorylation at specific sites like Y845 is known to regulate its signaling activity. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards phospho-EGFR (Y845) in human samples, making it an ideal choice for Western blot analysis.The phosphorylation of EGFR at Y845 is associated with enhanced receptor activation and downstream signaling cascades, ultimately impacting cell behavior and function.
Research into this post-translational modification of EGFR is crucial for understanding its role in various cellular processes, including cancer development and progression. The Phospho-EGFR (Y845) Polyclonal Antibody (CABP0023) facilitates the detection and analysis of phosphorylated EGFR at Y845, enabling in-depth investigations into the mechanisms underlying EGFR signaling and its implications in disease pathogenesis.
Product Name:
Phospho-EGFR-Y845 Rabbit Polyclonal Antibody
SKU:
CABP0023
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y845 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
200kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of A-431, using Phospho-EGFR-Y845 Rabbit pAb (CABP0023) at 1:2000 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 45s.
