The Phospho-EGFR Y845 Monoclonal Antibody is a crucial tool for researchers studying EGFR signaling pathways and their role in cancer development and progression. This antibody, generated through monoclonal technology, specifically targets the phosphorylated form of EGFR at the Y845 residue, a key site linked to cell proliferation, survival, and metastasis.The Phospho-EGFR Y845 Monoclonal Antibody is highly sensitive and specific, making it ideal for various applications such as Western blot, immunoprecipitation, and immunofluorescence. Its ability to detect the phosphorylated form of EGFR in human samples provides valuable insights into the activation status of this critical signaling protein.
Researchers in the fields of oncology, cell biology, and molecular biology will benefit from using this antibody to explore the mechanisms underlying EGFR-driven cancers and potential therapeutic interventions. By understanding the phosphorylation events that regulate EGFR activity, new avenues for targeted cancer therapies may be discovered, leading to improved outcomes for patients with EGFR-driven malignancies.
Product Name:
Phospho-EGFR (Y845) Monoclonal Antibody
SKU:
CABP1425
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho synthetic peptide corresponding to residues surrounding Y845 of Human EGFR.
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
200kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Clone Number:
ARC3122
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y845 (CABP1425) at 1:800 dilution.A-431 cells were treated by EGF (200 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
