The Phospho-EGFR (Y1197) Polyclonal Antibody (CABP0223) is a vital tool for researchers studying the activity of EGFR (Epidermal Growth Factor Receptor) phosphorylated at tyrosine 1197. This antibody, produced in rabbits, is highly specific for the phosphorylated form of EGFR in human samples, making it ideal for Western blot applications.EGFR is a key player in cell growth, proliferation, and differentiation, with dysregulation of its activity implicated in various cancers. Phosphorylation of EGFR at specific tyrosine residues, such as Y1197, can lead to downstream signaling cascades that promote tumor growth and survival.
The Phospho-EGFR (Y1197) Polyclonal Antibody allows for the detection and analysis of phosphorylated EGFR, enabling researchers to investigate the role of this protein in cancer development and progression.By understanding the mechanisms of EGFR phosphorylation at Y1197, researchers can gain insights into potential therapeutic targets for cancers driven by EGFR signaling. This antibody is a valuable tool for studies in oncology, molecular biology, and drug development, providing crucial information for the advancement of targeted cancer therapies.
Product Name:
Phospho-EGFR-Y1197 Rabbit Polyclonal Antibody
SKU:
CABP0223
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y1197 of human EGFR
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
170kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HUVEC cells, using Phospho-EGFR-Y1197 Rabbit pAb (CABP0223).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
