The Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody (CABP0992) is a cutting-edge research tool designed for studying the phosphorylation of EGFR at tyrosine 1173. EGFR, a key signaling molecule involved in cell growth and proliferation, is known to be activated through phosphorylation at different tyrosine residues, including Y1173. This antibody, raised in rabbits, exhibits high specificity and sensitivity for detecting phosphorylated EGFR at Y1173 in human samples.Validated for use in Western blot applications, the Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody (CABP0992) allows for precise detection and quantification of phosphorylated EGFR, providing researchers with valuable insights into signaling pathways involved in cancer development, progression, and resistance to therapy.
By targeting this specific phosphorylation site, researchers can gain a deeper understanding of EGFR signaling and its implications in cancer biology.With its unique ability to detect phosphorylated EGFR at Y1173, this antibody is an indispensable tool for investigations into EGFR signaling in cancer and other related fields. Its precise and reliable performance makes it an ideal choice for scientists studying the role of EGFR phosphorylation in disease pathogenesis and therapeutic targeting.
Product Name:
Phospho-EGFR-Y1173 Rabbit Monoclonal Antibody
SKU:
CABP0992
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y1173 of human EGFR
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Clone Number:
ARC1536
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of BxPC-3 cells, using Phospho-EGFR-Y1173 Rabbit mAb (CABP0992) at 1:1000 dilution.BxPC-3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
