The Phospho-EGFR (Y1172) Polyclonal Antibody (CABP0218) is a crucial tool for researchers studying the phosphorylation of the Epidermal Growth Factor Receptor (EGFR) at tyrosine 1172. This post-translational modification of EGFR is known to play a key role in signaling pathways involved in cell proliferation, survival, and migration.Our antibody is produced in rabbits and exhibits high reactivity with human samples, making it an ideal choice for Western blot applications. By specifically targeting the phosphorylated form of EGFR at Y1172, this antibody enables researchers to detect and analyze the activation status of EGFR in various cell types.
The phosphorylation of EGFR at tyrosine 1172 has been linked to cancer development and progression, making this antibody particularly valuable for studies in oncology. By gaining insights into the phosphorylation status of EGFR, researchers can unravel the mechanisms underlying tumor growth and potentially identify new therapeutic targets for cancer treatment.Overall, the Phospho-EGFR (Y1172) Polyclonal Antibody (CABP0218) offers a powerful tool for investigating the role of EGFR phosphorylation in cancer biology and holds great potential for advancing our understanding of cancer signaling pathways.
Product Name:
Phospho-EGFR-Y1172 Rabbit Polyclonal Antibody
SKU:
CABP0218
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y1172 of human EGFR
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of A-431, using Phospho-EGFR-Y1172 antibody (CABP0218) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
