The Phospho-EGFR (Y1148) Polyclonal Antibody (CABP0028) is a valuable tool for researchers studying the epidermal growth factor receptor (EGFR) and its phosphorylation at tyrosine 1148. EGFR is a key player in cell growth, proliferation, and survival, and dysregulation of its signaling pathways is implicated in various diseases, including cancer.This antibody, produced in rabbits, is highly specific for detecting phosphorylated EGFR at tyrosine 1148 in human samples. It has been validated for use in Western blot applications, allowing for the precise detection and quantification of phosphorylated EGFR in different cell types and tissues.
By targeting the phosphorylation of EGFR at this specific site, researchers can gain insights into the activation of EGFR signaling pathways and its role in various cellular processes. This antibody is particularly useful for studies in cancer research, as aberrant EGFR signaling is a common feature of many cancer types.Overall, the Phospho-EGFR (Y1148) Polyclonal Antibody is a valuable tool for investigating the role of EGFR phosphorylation in normal cellular function and disease states, providing researchers with a deeper understanding of EGFR signaling pathways and potential therapeutic targets for cancer and other diseases.
Product Name:
Phospho-EGFR-Y1148 Rabbit Polyclonal Antibody
SKU:
CABP0028
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y1148 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
180kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y1148 antibody (CABP0028) at 1:1000 dilution. A431 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
