The Phospho-EGFR (Y1086) Polyclonal Antibody (CABP0820) is a valuable tool for researchers studying the phosphorylation of EGFR at tyrosine 1086. EGFR, or Epidermal Growth Factor Receptor, is a protein involved in cell growth and proliferation, and phosphorylation at specific sites like Y1086 can regulate its activity and downstream signaling pathways. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of EGFR at Y1086, researchers can investigate the role of this post-translational modification in cancer development, progression, and treatment resistance.The phosphorylation of EGFR at Y1086 has been implicated in various cancer types, including lung cancer and glioblastoma, highlighting its potential as a therapeutic target.
Studying the phosphorylation status of EGFR can provide valuable insights into the mechanisms driving tumorigenesis and identify novel strategies for tackling drug resistance.Overall, the Phospho-EGFR (Y1086) Polyclonal Antibody is a reliable tool for researchers interested in understanding the complex signaling pathways involving EGFR phosphorylation and its implications in cancer biology. Its specificity and sensitivity make it an ideal choice for studies aiming to unravel the role of EGFR in cancer development and progression.
Product Name:
Phospho-EGFR-Y1086 Rabbit Polyclonal Antibody
SKU:
CABP0820
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y1086 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1086 Rabbit pAb (CABP0820) at 1:1000 dilution or EGFR antibody (CAB11351). A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
