The Phospho-EGFR (Y1068) Rabbit Monoclonal Antibody (CABP0994) is a high-quality antibody designed for research involving the epidermal growth factor receptor (EGFR), a key player in cell growth and development. This antibody, generated in rabbits, specifically recognizes the phosphorylated form of EGFR at the Y1068 site and is validated for use in various applications, including Western blot and immunohistochemistry.EGFR is a transmembrane protein that plays a crucial role in a variety of cellular processes, including cell proliferation, survival, and differentiation. Phosphorylation of EGFR at the Y1068 site is known to activate downstream signaling pathways involved in cell growth and survival, making this antibody an essential tool for studying EGFR signaling in cancer and other diseases.
The Phospho-EGFR (Y1068) Rabbit Monoclonal Antibody (CABP0994) is a valuable resource for researchers interested in investigating the role of EGFR signaling in cancer progression, drug resistance, and targeted therapy. Its high specificity and sensitivity make it a reliable tool for studying the molecular mechanisms underlying EGFR-related pathologies.
Product Name:
Phospho-EGFR-Y1068 Rabbit Monoclonal Antibody
SKU:
CABP0994
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y1068 of human EGFR (P00533).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Clone Number:
ARC1538
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of BxPC-3 cells, using Phospho-EGFR-Y1068 Rabbit mAb (CABP0994) at 1:1000 dilution.BxPC-3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
