The Phospho-EGFR (Y1068) Rabbit Monoclonal Antibody (CABP0027) is a powerful tool for research involving the epidermal growth factor receptor (EGFR), specifically phosphorylated at tyrosine 1068. This antibody, developed using rabbit monoclonal technology, is highly specific and sensitive for detecting phosphorylated EGFR in human samples. It has been validated for use in Western blot and immunohistochemistry applications, providing researchers with a reliable method for studying EGFR activation in various cell types.EGFR is a transmembrane receptor that plays a crucial role in cell proliferation, survival, and differentiation. Phosphorylation of EGFR at tyrosine 1068 is a key event in the receptor signaling cascade, leading to activation of downstream pathways involved in cell growth and survival.
Abnormal activation of EGFR signaling has been implicated in various types of cancer, making it a potential target for therapeutic interventions.By using the Phospho-EGFR (Y1068) Rabbit Monoclonal Antibody, researchers can gain insights into the activation status of EGFR in different cellular contexts, allowing for a better understanding of its role in cancer progression and response to therapy. This antibody is an essential tool for studies focused on EGFR signaling, offering high specificity and sensitivity for detecting phosphorylated EGFR in both basic research and clinical settings.
Product Name:
Phospho-EGFR-Y1068 Rabbit Monoclonal Antibody
SKU:
CABP0027
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y1068 of human EGFR (P00533).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
170kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Clone Number:
ARC0013
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1068 Rabbit mAb (CABP0027) at 1:1000 dilution.A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
