The Phospho-EGFR (Y1068) Polyclonal Antibody (CABP0301) is a valuable tool for researchers studying EGFR signaling pathways. EGFR, or epidermal growth factor receptor, is a transmembrane protein that plays a key role in cell growth and proliferation. The phosphorylation of tyrosine residue 1068 on EGFR is known to be important for downstream signaling cascades involved in cell survival and proliferation.This antibody, raised in rabbits, is highly specific for the phosphorylated form of EGFR at tyrosine 1068 and has been validated for use in various applications, including Western blot and immunofluorescence.
It enables detection and analysis of phospho-EGFR in diverse cell types and tissues, making it an ideal tool for studying the role of EGFR signaling in cancer, development, and other biological processes.Understanding the regulation of EGFR phosphorylation at tyrosine 1068 is critical for unraveling the complex signaling networks that control cell growth and survival. This antibody provides researchers with a reliable means of investigating the molecular mechanisms underlying EGFR activation and its implications for disease development and therapy.
Product Name:
Phospho-EGFR-Y1068 Rabbit Polyclonal Antibody
SKU:
CABP0301
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y1068 of human EGFR (NP_005219.2).
Sequence:
PEYI N
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:1000 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
185kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-EGFR-Y1068 Rabbit pAb (CAB11351).HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
