The Phospho-EGFR (T669) Polyclonal Antibody (CABP0025) is a valuable tool for researchers studying the EGFR signaling pathway, specifically phosphorylation on the T669 site. This antibody is raised in rabbits and is highly specific for human samples, making it ideal for use in Western blot applications.EGFR, or epidermal growth factor receptor, is a key player in cell growth, proliferation, and survival, and aberrant signaling through this pathway is implicated in various cancers. The phosphorylation of EGFR at the T669 site has been linked to the regulation of downstream signaling cascades, making it an important target for investigation in cancer biology research.
By using the Phospho-EGFR (T669) Polyclonal Antibody, researchers can detect and analyze the phosphorylation status of EGFR in various cell types, providing valuable insights into the role of this pathway in cancer development and progression. This antibody is a crucial tool for understanding the complex mechanisms of EGFR signaling and may lead to the development of targeted therapies for cancer treatment.
Product Name:
Phospho-EGFR-T669 Rabbit Polyclonal Antibody
SKU:
CABP0025
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T669 of human EGFR (NP_005219.2).
Sequence:
PLTP S
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
200kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 and HUVEC cells, using Phospho-EGFR-T669 Rabbit pAb (CABP0025) at 1:1000 dilution. A431 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. HUVEC cells were treated by EGF.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
