The Phospho-EGFR (S1070) Polyclonal Antibody (CABP0153) is a valuable tool for researchers studying the epidermal growth factor receptor (EGFR) signaling pathway, specifically phosphorylation at the S1070 residue. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the phosphorylated EGFR at S1070, this antibody facilitates the detection and analysis of EGFR activation in various cell types, making it an essential reagent for studies in cell signaling, cancer biology, and drug development.The EGFR is a key regulator of cell growth, proliferation, and survival, and aberrant activation of this receptor is commonly observed in various cancers. Phosphorylation of EGFR at specific sites, such as S1070, can have profound effects on downstream signaling pathways, leading to dysregulated cellular processes implicated in tumor development and progression.
Consequently, understanding the phosphorylation status of EGFR, including at S1070, is crucial for uncovering novel therapeutic targets and strategies for combating cancer.Overall, the Phospho-EGFR (S1070) Polyclonal Antibody (CABP0153) offers researchers a reliable and specific tool for investigating EGFR signaling dynamics and potential therapeutic interventions in cancer and other disease contexts. Its high reactivity and specificity make it an indispensable resource for advancing our understanding of EGFR biology and developing innovative
Product Name:
Phospho-EGFR-S1070 Rabbit Polyclonal Antibody
SKU:
CABP0153
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding S1070 of human EGFR
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of A-431, using Phospho-EGFR-S1070 antibody (CABP0153) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
