Anti-Phospho-EFNB1/EFNB2/EFNB3 (Tyr324) Antibody (PACO24401)

Antibody Name:	Phospho-EFNB1/EFNB2/EFNB3 (Tyr324) Antibody (PACO24401)
Antibody SKU:	PACO24401
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Peptide sequence around phosphorylation site of tyrosine 324 (G-D-Y(p)-G-H) derived from Human Ephrin B1/B2/B3 .

Form:	Liquid
Storage Buffer:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody.The lane on the right is treated with the antigen-specific peptide.
	Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody (left)or the same antibody preincubated with blocking peptide (right).

Background:	This gene encodes a member of the ephrin family. The encoded protein is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system.
Synonyms:	EPL2; CEL5-L; EFL-3; EFNB1; ELK-L

UniProt Protein Function:	EFNB1: a type I membrane protein of the ephrin family. A ligand of Eph-related receptor tyrosine kinases EphB1 and EphA1. Ephrins and ephrin receptors mediate numerous developmental processes, particularly in the nervous system. Ephrin-B1 may play a role in cell adhesion and functions in the development or maintenance of the nervous system. Binding to its receptor induces the collapse of commissural axons/growth cones in vitro. Induced by TNF-alpha. Expressed in brain, heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
UniProt Protein Details:	Protein type:Membrane protein, integral; Ligand, receptor tyrosine kinase Chromosomal Location of Human Ortholog: Xq12 Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; synapse; nucleus; lipid raft Molecular Function:protein binding; ephrin receptor binding Biological Process: axon guidance; cell-cell signaling; ephrin receptor signaling pathway; positive regulation of T cell proliferation; cell adhesion; embryonic pattern specification; neural crest cell migration Disease: Craniofrontonasal Syndrome
NCBI Summary:	The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008]
UniProt Code:	P98172
NCBI GenInfo Identifier:	1706668
NCBI Gene ID:	1947
NCBI Accession:	P98172.1
UniProt Secondary Accession:	P98172,D3DVU0,
UniProt Related Accession:	P98172
Molecular Weight:	346
NCBI Full Name:	Ephrin-B1
NCBI Synonym Full Names:	ephrin-B1
NCBI Official Symbol:	EFNB1
NCBI Official Synonym Symbols:	CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2
NCBI Protein Information:	ephrin-B1; EFL-3; LERK-2; ELK ligand; ligand of eph-related kinase 2; eph-related receptor tyrosine kinase ligand 2
UniProt Protein Name:	Ephrin-B1
UniProt Synonym Protein Names:	EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2
Protein Family:	Ephrin
UniProt Gene Name:	EFNB1
UniProt Entry Name:	EFNB1_HUMAN

Antibodies

Phospho-EFNB1/EFNB2/EFNB3 (Y324) Antibody (PACO02616)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)