The Phospho-EEF2K (S366) Polyclonal Antibody (CABP0845) is a valuable tool for researchers studying the phosphorylation of eukaryotic elongation factor 2 kinase (EEF2K) at serine 366. EEF2K is a key regulator of protein synthesis and cell growth, making it an important target for investigations into cancer, neurodegenerative diseases, and other conditions.This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically recognizing the phosphorylated form of EEF2K, researchers can gain crucial insights into the signaling pathways involved in protein translation and cell proliferation.
The phosphorylation of EEF2K at serine 366 has been linked to various cellular processes, including autophagy, apoptosis, and cell cycle regulation. Therefore, studying this post-translational modification can provide a deeper understanding of how EEF2K functions in health and disease.Overall, the Phospho-EEF2K (S366) Polyclonal Antibody is a valuable tool for researchers interested in elucidating the role of EEF2K phosphorylation in cellular processes, offering new insights that could drive the development of targeted therapies for diseases associated with dysregulated protein synthesis and cell growth.
Product Name:
Phospho-EEF2K-S366 Rabbit Polyclonal Antibody
SKU:
CABP0845
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1).
Sequence:
TLSG S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
eEF-2K; CaMKIII; HSU93850; Phospho-EEF2K-S366
Positive Sample:
HeLa,NIH/3T3,C6
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol, dendritic spine, glutamatergic synapse, postsynaptic density
Calculated MW:
82kDa
Observed MW:
110kDa
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
Purification Method:
Affinity purification
Gene ID:
29904
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-EEF2K-S366 Rabbit pAb (CABP0845) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
