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Phospho-EEF2K-S366 Rabbit Polyclonal Antibody (CABP0845)

SKU:
CABP0845
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 16 Anti-Phospho-EEF2K-S366 pAb Antibody CABP0845
  • Cell Biology Antibodies 16 Anti-Phospho-EEF2K-S366 pAb Antibody CABP0845
  • Cell Biology Antibodies 16 Anti-Phospho-EEF2K-S366 pAb Antibody CABP0845
€419
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Description

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Product Name:Phospho-EEF2K-S366 Rabbit Polyclonal Antibody
SKU:CABP0845
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1).
Sequence:TLSG S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:eEF-2K; CaMKIII; HSU93850; Phospho-EEF2K-S366
Positive Sample:HeLa,NIH/3T3,C6
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, dendritic spine, glutamatergic synapse, postsynaptic density
Calculated MW:82kDa
Observed MW:110kDa

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Purification Method:Affinity purification
Gene ID:29904
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-EEF2K-S366 Rabbit pAb (CABP0845) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

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