The Phospho-EEF2 (T56) Polyclonal Antibody (CABP0832) is specifically designed for research involving EEF2, a protein that plays a key role in protein synthesis regulation. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blotting applications. By binding to the phosphorylated form of EEF2 at threonine 56, this antibody enables precise detection and analysis of EEF2 in various cell types.Phosphorylation of EEF2 at threonine 56 is a critical post-translational modification that regulates the elongation stage of protein synthesis. Dysregulation of this phosphorylation event has been implicated in various diseases, including cancer and neurodegenerative disorders.
By studying the phosphorylation status of EEF2, researchers can gain insights into the molecular mechanisms underlying these diseases and identify potential therapeutic targets.Overall, the Phospho-EEF2 (T56) Polyclonal Antibody (CABP0832) is a valuable tool for studying the role of EEF2 phosphorylation in protein synthesis regulation and its implications in disease pathology. Its high specificity and sensitivity make it ideal for use in immunology, cancer research, and other fields where protein synthesis plays a crucial role.
Product Name:
Phospho-eEF2-T56 Rabbit Polyclonal Antibody
SKU:
CABP0832
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T56 of human Phospho-eEF2-T56 (NP_001952.1).
Sequence:
TRFT D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
EF2; EF-2; EEF-2; SCA26; Phospho-eEF2-T56
Positive Sample:
293T,293T+FBS
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
95kDa
Observed MW:
95kDa
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Purification Method:
Affinity purification
Gene ID:
1938
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-eEF2-T56 Rabbit pAb (CABP0832) at 1:3000 dilution.293T cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
